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Nucleotide polymorphism in chloroplast dna ofNicotiana debneyi

โœ Scribed by W. R. Scowcroft

Book ID
104709608
Publisher
Springer
Year
1979
Tongue
English
Weight
880 KB
Volume
55
Category
Article
ISSN
0040-5752

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โœฆ Synopsis

EcoR1 restriction endonuclease analysis of chloroplast DNA isolated from several distinct populations of Nicotiana debneyi has revealed a naturally occurring polymorphism. The chloroplast DNA of seven of the nine populations analysed possessed an additional EcoRl site. The origin of the additional restriction endonuclease fragments was confirmed by hybridisation of [(32) P]-cRNA to fractionated EcoRl restricted chloroplast-DNA fragments adsorbed to nitrocellulose filters. Reciprocal f1 hybrids between plants carrying the variant chloroplast-DNA's confirmed maternal inheritance of chloroplast-DNA.

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