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Nucleotide-dependent bending flexibility of tubulin regulates microtubule assembly

✍ Scribed by Wang, Hong-Wei; Nogales, Eva


Book ID
109894289
Publisher
Nature Publishing Group
Year
2005
Tongue
English
Weight
360 KB
Volume
435
Category
Article
ISSN
0028-0836

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Microtubule-associated proteins (MAPs) were separated from tubulin with several different methods. The ability of the isolated MAPs to reinduce assembly of phosphocellulose purified tubulin differed markedly between the different methods. MAPs isolated by addition of 0.35 M NaCl to taxol-stabilized