Nucleosomes, Histones & Chromatin Part A

✍ Scribed by Carl Wu and C. David Allis (Eds.)

• Publisher: Academic Press
• Year: 2012
• Tongue: English
• Leaves: 368
• Series: Methods in Enzymology 512
• Edition: 1
• Category: Library

✦ Synopsis

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins.

• Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field
• The first of 2 volumes covering nucleosomes, histones and chromatin
• Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins

✦ Table of Contents

Content:
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xi-xiii

Preface
Pages xv-xvi
Carl Wu, C. David Allis

Volume in series
Pages xvii-li

Chapter One - Examining Histone Posttranslational Modification Patterns by High-Resolution Mass Spectrometry
Pages 3-28
Shu Lin, Benjamin A. Garcia

Chapter Two - Investigating the Cell Cycle-Associated Dynamics of Histone Modifications Using Quantitative Mass Spectrometry
Pages 29-55
Mo Xu, She Chen, Bing Zhu

Chapter Three - A Method to Site-Specifically Incorporate Methyl-Lysine Analogues into Recombinant Proteins
Pages 57-69
Matthew D. Simon, Kevan M. Shokat

Chapter Four - Methods Applied to the Study of Protein Arginine Methylation
Pages 71-92
Donghang Cheng, Vidyasiri Vemulapalli, Mark T. Bedford

Chapter Five - Enzymatic Analysis of Tet Proteins: Key Enzymes in the Metabolism of DNA Methylation
Pages 93-105
Li Shen, Yi Zhang

Chapter Six - Peptide Microarrays to Interrogate the “Histone Code”
Pages 107-135
Scott B. Rothbart, Krzysztof Krajewski, Brian D. Strahl, Stephen M. Fuchs

Chapter Seven - Identifying Chromatin Readers Using a SILAC-Based Histone Peptide Pull-Down Approach
Pages 137-160
Michiel Vermeulen

Chapter Eight - High-Throughput Strategy to Identify Inhibitors of Histone-Binding Domains
Pages 161-185
Elise K. Wagner, Brittany N. Albaugh, John M. Denu

Chapter Nine - Validation of Histone-Binding Partners by Peptide Pull-Downs and Isothermal Titration Calorimetry
Pages 187-220
Kathryn Malecek, Alexander Ruthenburg

Chapter Ten - Analysis of Histone Chaperone Antisilencing Function 1 Interactions
Pages 223-241
Jean K. Scorgie, Douglas C. Donham III, Mair E.A. Churchill

Chapter Eleven - Quantifying Chromatin-Associated Interactions: The HI-FI System
Pages 243-274
Duane D. Winkler, Karolin Luger, Aaron R. Hieb

Chapter Twelve - Biochemical Assay for Histone H2A.Z Replacement by the Yeast SWR1 Chromatin Remodeling Complex
Pages 275-291
Gaku Mizuguchi, Wei-Hua Wu, Samar Alami, Ed Luk

Chapter Thirteen - Experimental Analysis of the Mechanism of Chromatin Remodeling by RNA Polymerase II
Pages 293-314
Daria A. Gaykalova, Olga I. Kulaeva, Nikolai A. Pestov, Fu-Kai Hsieh, Vasily M. Studitsky

Author Index
Pages 315-328

Subject Index
Pages 329-337