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Nucleosomal organization of chromatin in sperm nuclei of the bivalve molluscAulacomya ater

✍ Scribed by Carlos Olivares; Simon Ruiz


Book ID
104673612
Publisher
Springer
Year
1991
Tongue
English
Weight
510 KB
Volume
101
Category
Article
ISSN
0300-8177

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✦ Synopsis


The sperm nuclei of Aulacomya ater, family Mitylidae, contain three proteins (X, Aa5 and Aa6) which are specific to this cell type coexisting with a set of five somatic-type histones. Information about the chromatin structure resulting from this kind of association is scarce. Therefore, we have probed the structure of this sperm chromatin through digestion with micrococcal nuclease in combination with salt fractionation. The data obtained have allowed us to propose a nucleosomal arrangement for this chromatin. However, two types of nucleosomes would be present in agreement with their protein components.


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Chromatin organization in the sperm of the bivalve mollusks results from the interaction between a discrete number of protamine-like proteins (PL) and DNA. A small variable amount of histones is also present. An extensive study carried out on a relatively large number of species, within the class Bi