✦ LIBER ✦

Nucleosides and nucleotides. 162. Facile synthesis of 5′-5′-linked oligodeoxyribonucleotides with the potential for triple-helix formation

✍ Scribed by Yoshihito Ueno; Akira Ogawa; Aiko Nakagawa; Akira Matsuda

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 344 KB
Volume: 6
Category: Article
ISSN: 0960-894X
DOI: 10.1016/s0960-894x(96)00520-3

No coin nor oath required. For personal study only.

✦ Synopsis

The facile synthesis of 5'-5'-linked oligodeoxyribonucleotides (ODNs) with the potential for triple-helix formation is described. ODNs containing 5-trifluoroethoxycarbonyl-2'-deoxyuridine were treated with several diaminoalkanes to give ODNs carrying amino-linkers. After conversion of the amino-linkers in the ODNs to mercapto-linkers, two ODNs were linked by a disulfide bond to give 5'-5'-linked ODNs. The thermal stability of these ODNs with target duplexes was also studied.