Nucleoside und Nucleotide Teil 27 Festphasensynthese eines Tridecanucleosid-dodecaphosphats, das die unnatürliche Base 4-Amino-2(1H)-pyridinon enthält, und eines Octanucleosid-heptaphosphats zur Untersuchung der DNA-Polymerase-Spezifität
✍ Scribed by Roland Charczuk; Christoph Tamm
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- German
- Weight
- 454 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0018-019X
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✦ Synopsis
St. Johanns-Ring 19, CH-4056 Basel (23.1.87) Nucleosides and Nucleotides. Solid Phase Synthesis of a Tridecanucleoside Dodecaphosphate Containing the Unnatural Base 4-Amino-Z(lH)-pyridinone and of a Octanucleoside Heptaphosphate for the Determination of the DNA-Polymerase Specificity The tridecanucleoside dodecaphosphate d (T-T-Z-C-G-T-C-A-A-A-A-T-C) incorporating the modified nucleoside 4-amino-l-(2'-deoxy-~-~-ribofuranosyl)-2( lH)-pyridinone (Z,, 1) and the octdnucleoside heptaphosphate d (G-A-T-T-T-T-G-A) were synthesized by the solid-phase method using the triester method. The carrier material was a copolymer consisting of styrene with I % divinylbenzene.
HO 1 ') Tcil 26: [I]. 2, Abkiirzungen (vgl. auch [Zb]):v = (2-Chlorphenyl)-phosphat; (CNEt) = Cyanoethyl, [(MeO)*Tr] = 5'-0-Dimethoxytrityl; bz = Nh-Benzoyl; an = N4-Anisoyl; ib = N*-Isobutyryl; ac = N4-Acetyl.