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Nucleoside transport systems in Escherichia coli K12: Specificty and regulation

โœ Scribed by Agnete Munch-Petersen; Benete Mygind

Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
633 KB
Volume
89
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

Abstract

Two nucleoside transport systems have been verified and separated by mating and recombination experiments. The recipient strain was a mutant which is negative for transport of all nucleosides.

The two systems differ in specificity and in regulation. One system transports pyrimidine and adenine in specificity and in regulation. One system transports pyrimidine and adenine nucleosides, but not guanine nucleosides. It is regulated by the __cyt__R gene. The other system transports all nucleosides and is regulated by the __cyt__R as well as by the __deo__R genes.

Enzyme assays performed on whole cells of strains, able or unable to transport nucleosides, indicate that the nucleoside catabolizing enzymes are located inside the permeability barrier of the cell.

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