Nucleolus-organizer regions and heterochromatin in three species of Bovidae
โ Scribed by B. Mayr; E. Tesarik; H. Auer; H. Burger
- Publisher
- Springer Netherlands
- Year
- 1987
- Tongue
- English
- Weight
- 424 KB
- Volume
- 75
- Category
- Article
- ISSN
- 0016-6707
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โฆ Synopsis
Ag-NOR staining and a counterstain-enhanced fluorescence technique (chromomycin A3/distamycin A/DAPI staining = CDD-method) have been applied to ibex (Capra ibex L.), chamois (Rupicapra rupicapra L.) and bison (Bison bison L.) chromosomes. Chromomycin A3 visualization led to a well defined R-banding pattern along the chromosome arms and to a clear demonstration of centric heterochromatic bands of variable size. The nucleolus organizer regions (NORs) were found in the telomeric regions of the chromosomes 2, 3, 4, 5 and 28 of the ibex, of the chromosomes 1/3 (short arm), 2, 4, 5 and 28 of the chamois and of the chromosomes 2, 3, 4, 11 and 28 of the bison.
๐ SIMILAR VOLUMES
G-banding, C-banding and silver staining techniques were used to analyse the chromosomes of the roe deer (2n = 70, NF = 72). On the basis of several G-banded metaphases a standard description of the karyotype has been proposed. C-bands show constitutive heterochromatin in the centromeric regions of
Ag-staining of the nucleolus organizer region (NOR) was studied in the acrocentric chromosomes identified by Q-banding in repeated lymphocyte and skin fibroblast cultures from three different individuals. A similar pattern of Ag-stainability of NORs was found in the two tissues in each individual. S
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