The Burton Assay For Dna / Jaap H. Waterborg, Harry R. Matthews -- Daba Fluorescence Assay For Submicrogram Amounts Of Dna / Theodore Gurney, Elizabeth G. Gurney -- Preparation Of Rnase-free Dnase By Alkylation / Theodore Gurney, Elizabeth G. Gurney -- The Isolation Of Satellite Dna By Density Gradient Centrifugation / Craig A. Cooney, Harry R. Matthews -- The Isolation Of High Molecular Weight Eukaryotic Dna / C.g. Mathew -- Preparation Of Lyophilized Cells To Preserve Enzyme Activities And High Molecular Weight Nucleic Acids / Theodore Gurney -- Agarose Gel Electrophoresis Of Dna / Stephen A. Boffey -- Autoradiography Of Gels Containing 32p / Verena D. Huebner, Harry R. Matthews -- Detection Of Specific Dna Sequences : The Southern Transfer / C.g.p. Mathew -- The Extraction And Isolation Of Dna From Gels / Wim Gaastra, Per LinΓ₯ JΓΈrgensen --^ One-dimensional Electrophoresis Of Nucleic Acids In Agarose Using Denaturation With Formaldehyde And Identification Of 3h-labeled Rna By Fluorography / Theodore Gurney -- Gel Electrophoresis Of Rna In Agarose And Polyacrylamide Under Nondenaturing Conditions / R. Mcgookin -- The Extraction Of Total Rna By The Detergent And Phenol Method / Robert J. Slater -- Rna Extraction By The Proteinase K Method / R. Mcgookin -- Rna Extraction By The Guanidine Thiocyanate Procedure / R. Mcgookin -- The Purification Of Poly(a)-containing Rna By Affinity Chromatography / Robert J. Slater -- Messenger Rna Fractionation On Neutral Sucrose Gradients / R. Mcgookin -- The Estimation Of Mrna Content By Poly(u) Hybridization / Robert J. Slater -- Dna Directed In Vitro Protein Synthesis With Escherichia Coli S-30 Extracts / Jytte Josephsen, Wim Gaastra -- In Vitro Translation Of Messenger Rna In A Wheat Germ Extract Cell-free System / C.l. Olliver, A. Grobler-rabie, C.d. Boyd --^ In Vitro Translation Of Messenger Rna In A Rabbit Reticulocyte Lysate Cell-free System / C.l. Oliver, C.d. Boyd -- Immunoprecipitation Of In Vitro Translation Products With Protein A Bound To Sepharose / C.l. Olliver, C.d. Boyd -- In Vitro Continuation Of Rna Synthesis Initiated In Vivo / Theodore Gurney -- Synthesis Of Double-stranded Complementary Dna From Poly(a)+mrna / R. Mcgookin -- Plasmid Dna Isolation By The Cleared Lysate Method / Stephen A. Boffey -- Plasmid Dna Isolation (sheared Lysate Method) / J.w. Dale, P.j. Greenaway -- Small-scale Plasmid Dna Preparation / J.w. Dale, P.j. Greenaway -- Preparation Of Chromosomal Dna From E. Coli / J.w. Dale, P.j. Greenaway -- Preparation And Assay Of Phage Lambda / J.w. Dale, P.j. Greenaway -- Preparation Of Phage Lambda Dna / J.w. Dale, P.j. Greenaway -- The Use Of Restriction Endonucleases / Elliot B. Gingold -- Ligation Of Dna With T4 Dna Ligase / Wim Gaastra, Kirsten Hansen --^ The Use Of Alkaline Phosphatase To Prevent Vector Regeneration / J.w. Dale, P.j. Greenaway -- Bacterial Transformation / Elliot B. Gingold -- Bacterial Transformation (kushner Method) / J.w. Dale, P.j. Greenaway -- In Vitro Packaging Of Dna / J.w. Dale, P.j. Greenaway -- Yeast Transformation / Elliot B. Gingold -- Radiolabeling Of Dna By Nick Translation / C.g. Mathew -- Radiolabeling Of Dna Using Polynucleotide Kinase / Wim Gaastra, Jytte Josephsen -- Radiolabeling Of Dna With 3' Terminal Transferase / Wim Gaastra, Per Klemm -- Radiolabeling Of Dna With The Klenow Fragment Of Dna Polymerase / Wim Gaastra, Jytte Josephsen -- Identification Of Recombinant Plasmids By In Situ Colony Hybridization / J.w. Dale, P.j. Greenaway -- Identification Of Recombinant Phages By Plaque Hybridization / J.w. Dale, P.j. Greenaway -- Plasmid Screening Using Single Colony Lysates / J.w. Dale, P.j. Greenaway -- Immunological Detection Of Gene Expression In Recombinant Clones / J.w. Dale, P.j. Greenaway --^ The Isolation Of Minicells / Wim Gaastra, Per Klemm -- The Isolation Of Maxicells / Wim Gaastra, Per Klemm -- The Identification Of Gene Products In Minicells And Maxicells / Wim Gaastra, Per Klemm -- Molecular Cloning In Bacteriophage Lambda And In Cosmids / Claus Christiansen -- Dna Transformation Of Mammalian Cells / Jeffrey W. Pollard, Yunus Luqmani, Alan Bateson, Kokila Chotai -- Chemical Cleavage (maxam And Gilbert) Method For Dna Sequence Determination / Wim Gaastra -- Gel Electrophoretic Analysis Of Dna Sequencing Products / Wim Gaastra -- Dna Sequence Determination Using Dideoxy Analogs / M.j. Owen. Edited By John M. Walker. Includes Bibliographical References And Index.