✦ LIBER ✦

Nucleic acid spot hybridization with nonradioactive labeled probes in screening for human papillomavirus DNA sequences

✍ Scribed by Ronald Melki; Bernadette Khoury; Francois Catalan

Book ID: 102377955
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 432 KB
Volume: 26
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890260205

No coin nor oath required. For personal study only.

✦ Synopsis

We examined 161 human tissue samples using the spot hybridization technique with nonradioactive labeled DNA probes of human papillomavirus (HPV). Whole cells were spotted on nitrocellulose filters; DNA of the cells was denatured and fixed to the filter. Then the DNA spots were hybridized to nonradioactive labeled DNA and monitored by a sandwich immunoenzymatic reaction. This technique is simple, sensitive, specific, requires no special equipment, and can be used in clinical settings. HPV DNA was found in 92% of samples in which, on the basis of histologic and colposcopic criteria, its presence was suspected, as well as in 31 samples where it was not suspected.

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