Nucleic acid sensor for insecticide detection

✍ Scribed by Pratima R. Solanki; Nirmal Prabhakar; M. K. Pandey; B. D. Malhotra

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 309 KB
• Volume: 21
• Category: Article
• ISSN: 0952-3499
• DOI: 10.1002/jmr.888

✦ Synopsis

Abstract

Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO^−^~4~)‐doped PANI film deposited onto indium‐tin‐oxide (ITO) glass plate using 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride (EDC)/N‐hydroxysuccinimide (NHS) chemistry. These dsCT‐DNA‐PANI‐ClO~4~/ITO and PANI‐ClO~4~/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier‐transform‐infrared (FTIR) measurements. This disposable dsCT‐DNA‐PANI‐ClO~4~/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005 ppm) and trichlorfon (0.01 ppm) in 30 and 60 s, respectively. Copyright © 2008 John Wiley & Sons, Ltd.