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Nucleic acid hybridization for detection of herpes viruses in clinical specimens

✍ Scribed by Volker Schuster; Bertfried Matz; Helga Wiegand; Axel Polack; Brigitte Corsten; Dieter Neumann-Haefelin

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
662 KB
Volume
19
Category
Article
ISSN
0146-6615

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✦ Synopsis

A diagnostic hybridization assay for detecting varicella zoster virus (VZV), herpes simplex virus (HSV), and Epstein-Barr virus (EBV) in different clinical specimens was developed using cloned viral DNAs as probes. All probes detected at least 5 pg of homologous DNA and did not cross-react with other viral or cellular DNA. Results of cell culture, serology, and DNA assay were highly concordant. Using a simple standardized protocol for preparation of specimens, hybridization, and washing procedures, this sensitive and specific assay appears to be useful for screening clinical specimens and may be helpful in confirming the serological diagnosis of HSV encephalitis and persistent EBV infections or EBV-associated diseases.

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