✦ LIBER ✦
Nucleic acid chromatography: Substitute solid support material for RPC-5 columns
✍ Scribed by J.M. Flanagan; R.K. Fujimura; K. Bruce Jacobson
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 523 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Chromatography of tRNA and DNA fragments on columns of reverse-phase 5 (RPC-5) exchange material has been widely employed for analytical and preparative studies. The Plaskon bead that formed the solid support on which a quaternary amine was absorbed is no longer commercially available. A Voltalef bead is available and provides similar, though not identical, chromatograms for Asp-tRNA, Ser-tRNA, and certain DNA fragments. Procedures are described for preparation of the column packing and for long-term operation of the column.