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Nucleic acid chemistry: Resolution of supercoiled, intact relaxed, and nicked double-stranded DNAs by formamide-sucrose gradients

✍ Scribed by C.Neal Ellis; Sharon Conley-Hixon; William S. Blakemore


Book ID
102628672
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
286 KB
Volume
163
Category
Article
ISSN
0003-2697

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✦ Synopsis


A technique which will separate superhelical, intact nonsuperhelical, and damaged mitochondrial DNA molecules by use of sedimentation gradients of formamide-sucrose is described. This technique can be used to detect damage to the mitochondrial DNA helix. @ 1987 Academic Press, Inc.