Nuclear transplantation in sheep embryos
โ Scribed by Willadsen, S. M.
- Book ID
- 109742953
- Publisher
- Nature Publishing Group
- Year
- 1986
- Tongue
- English
- Weight
- 390 KB
- Volume
- 320
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/320063a0
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The ability of foreign nuclei to support development in nuclear transplantation manipulations has proven an effective means to assess the consequences of nuclear differentiation. In addition, nuclear transplantation might serve to define the persistence and role of maternally inherited cytoplasmic c
The nuclei from one-, two-, four-, and eight-cell rat embryos were transferred into enucleated zygotes and development to term of the reconstituted embryos, after transfer to recipients, was examined. The rate of electrofusion of enucleated zygotes with karyoplasts was quite high (81-92%), but the f
## Abstract The transfer of nuclei from cleavage stage embryos to enucleated activated meiotic metaphase II oocytes results in a reprogramming of the transferred nucleus such that it behaves as a zygotic nucleus. One estimator of nuclear reprogramming is nuclear swelling after nuclear transfer. The