Nuclear-targeted minicircle to enhance gene transfer with non-viral vectors in vitro and in vivo

✍ Scribed by Laurence Vaysse; Lisa G. Gregory; Richard P. Harbottle; Eric Perouzel; Oleg Tolmachov; Charles Coutelle

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 333 KB
• Volume: 8
• Category: Article
• ISSN: 1099-498X
• DOI: 10.1002/jgm.883

✦ Synopsis

Abstract

Background

To develop more efficient non‐viral vectors, we have previously described a novel approach to attach a nuclear localisation signal (NLS) to plasmid DNA, by generating a fusion protein between the tetracycline repressor protein TetR and an SV40 NLS peptide (TetR‐NLS). The high affinity of TetR for the DNA sequence tetO is used to bind the NLS to DNA. We have now investigated the ability of this system displaying the SV40 NLS or HIV‐1 TAT peptide to enhance nuclear import of a minimised DNA construct more suitable for in vivo gene delivery: a minicircle.

Methods

We have produced a new LacZ minicircle compatible with the TetR system. After transfection of the minicircle in combination with TetR‐NLS or TetR‐TAT using different transfection agents, we first measured β‐galactosidase activity in vitro. We then used a special delivery technique, in which DOTAP/cholesterol liposomes and DNA/protein complexes are sequentially injected intravenously, to evaluate the activity of this system in vivo.

Results

In vitro results showed a 30‐fold increase in transfection efficiency of the nuclear‐targeted minicircle compared to normal plasmid lipofection. Results on cell cycle arrested cells seem to indicate a different mechanism between the TetR‐NLS and TetR‐TAT. Finally, we demonstrate a more than 6‐fold increase in β‐galactosidase expression in the mouse lung using the minicircle and the TetR‐TAT protein. This increase is specific for the peptide sequence and is not observed with the control protein TetR.

Conclusions

Our results indicate that the combination of a minicircle DNA construct with a TetR nuclear‐targeting system is able to potentiate gene expression of non‐viral vectors. Copyright © 2006 John Wiley & Sons, Ltd.