Nuclear Reprogramming: Methods and Protocols

✍ Scribed by Kejin Hu

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 310
• Series: Methods in Molecular Biology 2239
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xi
Pig Cloning Using Somatic Cell Nuclear Transfer (Hongsheng Ouyang, Jianyong Han, Yongye Huang)....Pages 1-18
Cloning of Monkeys by Somatic Cell Nuclear Transfer (Shiyu Sun, Zhaodi Liao, Zhen Liu, Qiang Sun)....Pages 19-31
Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors (Emre Bektik, Ji-Dong Fu)....Pages 33-46
Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media (Xiaowen Wang, Conrad P. Hodgkinson, Victor J. Dzau)....Pages 47-59
Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-Free Cells (Minhyung Lee, Janghwan Kim, Rajesh Ambasudhan)....Pages 61-75
Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts (Victoria A. Church, Kitra Cates, Lucia Capano, Shivani Aryal, Woo Kyung Kim, Andrew S. Yoo)....Pages 77-100
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium (Zhicheng Shao, Ricardo Raúl Cevallos, Kejin Hu)....Pages 101-116
Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency (Wei Wen, Tao Cheng, Xiao-Bing Zhang)....Pages 117-133
Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells (Fabia Febbraro, Muwan Chen, Mark Denham)....Pages 135-151
Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling (Chun Liu, Mohamed Ameen, Sukaina Himmati, Dilip Thomas, Nazish Sayed)....Pages 153-162
Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA (Robert R. Annand)....Pages 163-174
Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators (Jere Weltner, Ras Trokovic)....Pages 175-198
Reprogramming Porcine Fibroblast to EPSCs (Xuefei Gao, Degong Ruan, Pentao Liu)....Pages 199-211
Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance (Jennifer L. Brehm, Molly M. Miles, Katie S. Remondini, Tenneille E. Ludwig)....Pages 213-234
Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers (Ricardo Raúl Cevallos, Md Emon Hossain, Ruowen Zhang, Kejin Hu)....Pages 235-249
G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures (Erik McIntire, Kimberly Leonhard, Seth Taapken, Anna Lisa Larson)....Pages 251-268
Become Competent in Generating RNA-Seq Heat Maps in One Day for Novices Without Prior R Experience (Kejin Hu)....Pages 269-303
Back Matter ....Pages 305-306

✦ Subjects

Life Sciences; Nucleic Acid Chemistry; Stem Cells