Nuclear matrix proteins ofPhysarum polycephalum

✍ Scribed by B. Kowalska-Loth; K. Staron

• Book ID: 104768103
• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 391 KB
• Volume: 11
• Category: Article
• ISSN: 0301-4851
• DOI: 10.1007/bf00419600

✦ Synopsis

The proteins of nuclear matrix preparations from Physarum polycephalum were compared with analogous mammalian fractions by gel electrophoresis, DNA-binding studies and immunological tests. Polypeptides of 28 and 36 K dalton, which dominate in Physarum preparations, differed from calf thymus matrix proteins in that they were basic and showed low affinity to DNA. These polypeptides were present at about 1.2 mg per mg of nuclear DNA. Polypeptides of higher molecular weight occurred in the preparation at about 0.5 mg per mg of nuclear DNA. At least some of the latter proteins showed high affinity to DNA and crossreacted with the antiserum against calf thymus matrix proteins.