Nuclear magnetic resonance spectra of heparin in admixture with dermatan sulfate and other glycosaminoglycans. 2-D spectra of the chondroitin sulfates
✍ Scribed by Kevin R. Holme; Arthur S. Perlin
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 929 KB
- Volume
- 186
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Characteristics
of the rH-n.m.r. spectra of heparin admixed with other glycosaminoglycans are described with respect to the identification of the latter as possible contaminants of pharmaceutical heparins. Chemical shift differences are sufficiently large, particularly with the aid of resolution enhancement, to allow for the detection of dermatan sulfate, chondroitin 4-or 6-sulfate, h$luronic acid, or heparan sulfate as a minor constituent in the presence of heparin. The acetamidomethyl resonance region (6 1.95-2.15) is especially useful in this context, both for identification and quantitative estimation. Whereas dermatan sulfate is a common contaminant of pharmaceutical heparin preparations, in some instances comprising lO-15 percent of the polymer mixture, the other glycosaminoglycans, by contrast, were not detected in such preparations.
Two-dimensional heterocorrelation and homocorrelation n.m.r. experiments have provided 'H-and 13C-chemical shift data that complete or verify (or both) previous information available for heparin, dermatan sulfate, and chondroitin 4-and 6-sulfates (chondroitins A and C).