Nuclear magnetic resonance evidence for radiation damage of saturated phosphatidylcholine in bilayers

✍ Scribed by Mariano Casu; Adolfo Lai; Gianni Erriu; Salvatore Onnis; Nazario Zucca

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 321 KB
• Volume: 30
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1260300510

✦ Synopsis

Abstract

Multilayer membranes of 1,2‐dipalmitoyl‐sn‐glycero‐3‐phosphorylcholine were prepared in D~2~O and exposed to γ‐radiation. Changes in the chemical structure due to irradiation were studied by ^1^H, ^13^C and ^31^P NMR after lyophilization and dissolution in chloroform‐methanol (1 : 2, v/v). One‐ and two‐dimensional techniques were used. 1‐Palmitoyl‐sn‐glycero‐3‐phosphorylcholine, 2‐palmitoyl‐sn‐glycero‐3‐phosphorylcholine, glycero‐3‐phosphorylcholine and palmitic acid were identified as new components. Their development during irradiation was quantitatively determined. The results were discussed in terms of possible reaction steps responsible for the radiolytic decomposition.