๐”– Bobbio Scriptorium
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Nuclear and cytoplasmic localization of different basic fibroblast growth factor species

โœ Scribed by M. Renko; N. Quarto; T. Morimoto; D. B. Rifkin

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
783 KB
Volume
144
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

The subcellular distribution of basic fibrobla5tic growth factor (bFGF) was analyzed by subcellular fractionation and iinmunofluorescence to gain insight into potential mechanisms for its release from cells. Subcellular fractionation of either SK-Hep-I cells or NIH 3T3 cells transfected with a bFGF cDNA revealed that the 18 kd form of bFGF was found primarily in the cytosolic fraction, whereas the 22 and 24 kd forms of bFGF were found preferentially in ribosomal and nuclear fractions. Analysis of bFGF distribution by imrnunofluorescence using an antibody that recognized all forms of bFCF indicated both cytoplasmic and nuclear localization but failed to reveal any growth factor in structures representing secretory vesicles. Therefore, bFCF has a distribution inconsistent with that of a secretory protein.

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