Genetic manipulation to identify limitin
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C. Perry Chou; Chih-Chang Yu; Jen-Hung Tseng; Ming-I Lin; Hua-Kuo Lin
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Article
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1999
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John Wiley and Sons
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English
โ 283 KB
We have identified the bottleneck steps limiting expression of penicillin acylase (PAC) through comparison of the expression performance for various PACexpression vectors constructed by genetically modulating the efficiencies of transcription and/or translation of the pac gene. To our knowledge, thi