Novel Sxra ES cell line offers hope for Y chromosome gene-targeted mice
✍ Scribed by Elizabeth M. Simpson; Kevin A. Johnson; Bobbi-Jo Shirley; Ge Y. Fang; Janet K. Bayleran; Charles P. Lerner
- Book ID
- 102222145
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 342 KB
- Volume
- 33
- Category
- Article
- ISSN
- 1526-954X
No coin nor oath required. For personal study only.
✦ Synopsis
A mouse targeted for a Y Chromosome gene has not been reported. Because the Y Chromosome is present in only one copy, and most of its genes are critical for germ cell development, such a mouse would likely be infertile. Thus, we describe a new reproductive strategy to enable transmission of targeted Y Chromosome genes to subsequent generations. The strategy uses two segregating copies of Y Chromosome genes to mimic the autosomal condition. To achieve this, we developed a new embryonic stem cell line from the XYSxr(a) mouse, which carries a duplication of the gene-rich Y Chromosome short arm. Importantly, we demonstrate germ line transmission of the YSxr(a) chromosome and describe this significant new tool as a practical solution to enable reproduction in mice targeted for Y Chromosome genes.