Applicability of modern microfabrication technology to electrophoresis microchips initiated a rapidly moving interdisciplinary field in analytical chemistry. Electric field mediated separations in microfabricated devices (electrophoresis microchips) are significantly faster than conventional gel ele
Novel quantitative electrophoretic analysis of endotoxins on microchips
✍ Scribed by Anikó Kilár; Viktor Farkas; Krisztina Kovács; Béla Kocsis; Ferenc Kilár
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 354 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
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Protein cysteine residues were labeled with the thiolspecific fluorescent probe monobromobimane prior to electrophoresis. Labeling was rapid and complete under the conditions employed. Scanning the negative films of photographed gels with a laser densitometer provided quantitation. Analysis of five