Novel Poly(amidoamine)s with Pendant Primary Amines as Highly Efficient Gene Delivery Vectors
✍ Scribed by Min Liu; Jun Chen; Yan-Ping Cheng; Ya-Nan Xue; Ren-Xi Zhuo; Shi-Wen Huang
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 554 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
Three poly(amidoamine)s with pendant primary amines were synthesized by Michael polyaddition of a diamine to __N,N‐__methylenebis(acrylamide). The poly(amidoamine)s with primary and tertiary amines possess a high buffer capacity between pH = 5 and 7, which facilitates the escape of polymer/DNA complexes from endosomes according to the ‘proton sponge hypothesis’. In vitro cytotoxicity of poly(amidoamine)s depends on the side‐chain structure of the polymer. Polymers 1a–1c can condense plasmid DNA by electrostatic interactions to form nanosized polyelectrolyte complexes with a positive surface charge. In vitro transfection results indicate that the transfection efficiency of polymer 1b under optimized conditions is comparable to that of commercial branched PEI 25 000.
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