Novel Plexor™ SNP genotyping technology: comparisons with TaqMan® and homogenous MassEXTEND™ MALDI-TOF mass spectrometry
✍ Scribed by E.A. Tindall; G. Speight; D.C. Petersen; E.J.D. Padilla; V.M. Hayes
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 313 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1059-7794
No coin nor oath required. For personal study only.
✦ Synopsis
Analysis of SNPs for association, linkage, haplotype, and pharmacogenetic studies has led to a dramatic increase in the number and evolution of medium-to high-throughput genotyping technologies. This study introduces Plexor TM as a new method for medium-throughput (single SNP) genotyping. We compare this fluorescent-based chemistry for call rate, accuracy, affordability, throughput, and overall efficiency against two commonly used technologies. These include fluorescent-based TaqMan s allelic discrimination for single SNP analysis (medium-throughput) and the homogenous MassEXTEND TM (hME TM ) chemistry using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for multiple SNP analysis (highthroughput). Analysis of 11 SNPs, including all six possible nucleotide substitutions, showed Plexor TM to be highly comparable for both call rate (94.7%) and accuracy (99.2%) to the TaqMan s (94.6% and 99.8%, respectively) and hME TM (91.9% and 98.1%, respectively) chemistries. We demonstrate that this novel method is an efficient, cost-effective alternative to TaqMan s genotyping commonly used in diagnostic settings.