Novel Oligonucleotide Carrier as Scavenger for Reactive Oxygen Species

✍ Scribed by Yutaka Ikeda; Rie Suzuki; Toru Yoshitomi; Yukio Nagasaki

• Book ID: 102467419
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 482 KB
• Volume: 11
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201000305

✦ Synopsis

Abstract

A novel oligonucleotide carrier which can scavenge ROS is described. The synthesized graft polymer is composed of a PEG segment and a TEMPO‐containing hydrophobic segment for scavenging ROS. This graft polymer can form a PIC through electrostatic interaction with oligonucleotides such as siRNA. The amount of ROS was monitored by fluorescence measurements using H~2~DCFDA as a probe, and it was confirmed that the ROS level was effectively suppressed. The cellular uptake of PIC containing the fluorescence‐labeled oligonucleotide was evaluated by fluorescence microscopy. Delivered siRNA suppressed the expression of the mRNA. The prepared graft copolymer is thus a promising candidate as a novel oligonucleotide carrier which also reduces ROS damage generated by cationic polymer carriers. magnified image