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Novel non-viral vectors for gene delivery: Synthesis of a second-generation library of mono-functionalized poly-(guanidinium)amines and their introduction into cationic lipids

✍ Scribed by Gerardo Byk; Javier Soto; Christophe Mattler; Marc Frederic; Daniel Scherman


Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
111 KB
Volume
61
Category
Article
ISSN
0006-3592

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✦ Synopsis


The development of new gene delivery technologies is a prerequisite towards gene therapy clinical trials. Because gene delivery mediated by viral vectors remains of limited scope due to immunological and propagation risks, the development of new non-viral gene delivery systems is of crucial importance. We have synthesized a secondary library of mono-functionalized poly-(guanidinium)amines generated from a library of mono-functionalized polyamines applying the concept of ''libraries from libraries.'' The method allows a quick and easy access to mono-functionalized geometrically varied poly-(guanidinium)amines. The new building blocks were introduced into cationic lipids to obtain novel poly-(guanidinium)amine lipids, which are potential DNA vectors for gene delivery.