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Novel method using antibiotics for the determination of the rate-limiting step in the secretion pathway of glucoamylase from recombinant yeast

✍ Scribed by Hyung Joon Cha; Young Je Yoo

Publisher: Springer-Verlag
Year: 1996
Tongue: English
Weight: 673 KB
Volume: 10
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00184025

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✦ Synopsis

A novel method for the determination of the rate-limiting step in the protein secretion pathway in recombinant strains of Saccharomyces cerevisiae is proposed which used several antibiotics that specifically inhibit the processing enzymes in the secretion pathway. This novel method has advantages such as the relative simplicity of the experiments, non-requirement of secretion mutants, and absence of strain effects.

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