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Novel method for NMR spectral correlation between the native and the denatured states of a protein. Application to ribonuclease A

✍ Scribed by Akasaka, Kazuyuki; Naito, Akira; Imanari, Mamoru

Book ID
126023793
Publisher
American Chemical Society
Year
1991
Tongue
English
Weight
294 KB
Volume
113
Category
Article
ISSN
0002-7863

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A novel method useful for selective isolation of the C-terminal peptide from a tryptic digestion mixture of a protein has been developed by taking advantage of a unique property of anhydrotrypsin, which has a strong specific affinity for the peptides containing arginine or lysine at their C-termini.