Novel mechanism of tumorigenesis: Increased transforming growth factor-?1 suppresses the expression of connexin 43 in BALB/cJ mice after implantation of poly-L-lactic acid
✍ Scribed by Ahmed, Saifuddin ;Tsuchiya, Toshie
- Book ID
- 102294877
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 121 KB
- Volume
- 70A
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Abstract
Poly‐L‐lactic acid (PLLA) is a widely used promising material for surgical implants such as tissue‐engineered scaffolds. In this study, we aimed to determine the in vivo effect of PLLA plates on the cellular function of subcutaneous tissue in the two mouse strains, BALB/cJ and SJL/J, higher and lower tumorigenic strains, respectively. Gap‐junctional intercellular communication (GJIC) and the expression of connexin 43 (Cx43) protein were significantly suppressed, whereas the secretion of transforming growth factor‐β1 (TGF‐β1) level was significantly increased in PLLA‐implanted BALB/cJ mice compared with BALB/cJ controls. However, no significant difference in TGF‐β1 secretion was observed between the SJL/J‐implanted and SJL/J control mice. We found for the first time that a significant difference was observed between the two strains; thus, the PLLA increased the secretion of TGF‐β1 and suppressed the mRNA expression of Cx43 at the earlier stage after implantation into the higher‐tumorigenic strain, BALB/cJ mice. This novel mechanism might have a vital role in the inhibition of GJIC and promote the tumorigenesis in BALB/cJ mice. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 335–340, 2004