✦ LIBER ✦
Novel insights into the use of Glowing LNA as nucleic acid detection probes—Influence of labeling density and nucleobases
✍ Scribed by Michael E. Østergaard; Jyotirmoy Maity; B. Ravindra Babu; Jesper Wengel; Patrick J. Hrdlicka
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 314 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0960-894X
No coin nor oath required. For personal study only.
✦ Synopsis
Appropriately designed 2 0 -N-(pyren-1-yl)carbonyl-2 0 -amino-LNA (locked nucleic acid) display large increases in fluorescence intensity and remarkably high quantum yields upon hybridization with nucleic acid targets. Thermal denaturation and fluorescence spectroscopy studies on ONs modified with known thymine monomer X and novel 5-methylcytosine monomer Y provide new insights into the design principles and mechanism of these Glowing LNA nucleic acid detection probes.