To determine whether the isoform of UDP-glucuronosyltransferase which catalyzes the formation of bilirubin monoglucuronide also mediates the formation of bilirubin diglucuronide and other specific sugar conjugates of billrubin, Wistar rats were treated with clofibrate (300 mg per kg i.p. x 7 days);
Novel inhibitors and substrates of bilirubin: UDP-glucuronosyltransferase Arylalkylcarboxylic acids
✍ Scribed by Sylvie FOURNEL-GIGLEUX; Steven R. P. SHEPHERD; Marie-Christiane CARRE; Brian BURCHELL; Gérard SIEST; Paul CAUBERE
- Book ID
- 115127627
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 905 KB
- Volume
- 183
- Category
- Article
- ISSN
- 1432-1327
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A rapid and sensitive radioassay for measuring UDP-glucuronosyltransferase activities (EC 2.4.1.17) toward the major endogenous substrates hyodeoxycholic and hyocholic acids, bilirubin, estriol, androsterone, and testosterone has been developed. In this assay, 14C-labeled glucuronides are formed fro
The activity of UDP-glucuronosyltransferase (UDPGT) and the concentration of its endogenous substrate, 5'-diphosphoglucuronic acid (UDPGA), have been measured in human liver, kidney, lung and intestinal mucosa. The activity of UDPGT was tissue- and substrate-dependent. The liver/kidney and liver/int