Novel function of TWEAK in inducing intervertebral disc degeneration

✍ Scribed by Masanori Wako; Hirotaka Haro; Takashi Ando; Kyosuke Hatsushika; Tetsuro Ohba; Sadahiro Iwabuchi; Atsuhito Nakao; Yoshiki Hamada

• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Weight: 396 KB
• Volume: 25
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.20445

✦ Synopsis

Abstract

The goal of this research was to examine the role of TWEAK in normal disc cells and to investigate its potential role in disc degeneration. We performed histological examinations of disc tissues and assessed the role of the novel cytokine TWEAK using murine organ disc culture. The expression of both TWEAK and its receptor, Fn14, in discs was confirmed by immunohistochemistry and quantitative real‐time PCR. TWEAK induced disc cells to generate MMP‐3 in a dose‐ and time‐dependent manner. This induction was strongly inhibited in the presence of a neutralizing antibody to TWEAK or a chimeric Fn14/Fc fusion protein. In disc tissues derived from TNF‐α receptor 1‐ or TNF‐α receptor 2‐deficient mice, recombinant TWEAK modestly induced MMP‐3. In contrast, in disc cultures lacking TWEAK, tissues from wild‐type mice or receptor‐deficient mice failed to express MMP‐3. Furthermore, aggrecan expression was potently abrogated in a time‐dependent manner in the presence of recombinant TWEAK. This is the first report to confirm expression of TWEAK and its receptor Fn14 in murine intervertebral disc tissues. The data suggest that TWEAK plays a role in MMP‐3 up‐regulation and aggrecan down‐regulation in disc tissues, resulting in proteoglycan degradation and promotion of disc degeneration. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:1438–1446, 2007