Novel explant model to study mechanotransduction and cell–cell communication

✍ Scribed by C. Edward Hoffler; Kurt D. Hankenson; Joshua D. Miller; Sukhinderdeep K. Bilkhu; Steven A. Goldstein

• Book ID: 102395481
• Publisher: Elsevier Science
• Year: 2006
• Tongue: English
• Weight: 482 KB
• Volume: 24
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.20207

✦ Synopsis

Abstract

To understand in situ behavior of osteocytes, we characterized a model of osteocytes in their native bone matrix and demonstrated real‐time biologic activity of osteocytes while bending the bone matrix. Using 43 male Sprague‐Dawley rats, dumbbell‐shaped explants were harvested from stainless steel femoral implants after 6–12 weeks and incubated in culture medium or fixed. Sixteen specimens were used to determine bone volume density (BV/TV), volumetric bone mineral density (BMD) and histology for different implantation periods. Osteocyte viability was evaluated by L‐lactate dehydrogenase (LDH) activity in 12 cultured explants. Confocal microscopy was used to assess tracer diffusion in three explants and changes in osteocyte pH of a mechanically loaded explant. From 6 to 12 weeks, explant BV/TV and volumetric BMD trended up 92.5% and 101%, respectively. They were significantly and highly correlated. Tissues were uniformly intramembranous and all bone cell types were present. Explants maintained LDH activity through culture day 8. Diffusion at 200 µM was limited to 1,209 Da. Explants appeared capable of reproducing complex bone biology. This model may be useful in understanding osteocyte mechanotransduction in the context of a physiologically relevant bone matrix. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 24:1687–1698, 2006