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Novel design of multicapillary arrays for high-throughput DNA sequencing

✍ Scribed by Andriy Tsupryk; Michael Gorbovitski; Evgeni A. Kabotyanski; Vera Gorfinkel

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 619 KB
Volume: 27
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200500594

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✦ Synopsis

Abstract

A novel approach to design and optimize linear multicapillary arrays (LMCAs) for high‐throughput DNA sequencing is proposed. A significant increase in the number of capillary lanes is obtained due to the use of composite insertions alternately placed between working capillaries of the array and a specific combination of refractive indices of the DNA separation matrix, capillary glass, the insertions and a medium which surrounds the capillary array. Theoretical and experimental studies showed that in conjunction with a dual‐side laser illumination scheme, the proposed LMCA design allows a simultaneous uniform irradiation of as many as 550 working capillaries.

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