Novel complexation hydrogels for oral peptide delivery:In vitro evaluation of their cytocompatibility and insulin-transport enhancing effects using Caco-2 cell monolayers
✍ Scribed by Ichikawa, Hideki ;Peppas, Nicholas A.
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 164 KB
- Volume
- 67A
- Category
- Article
- ISSN
- 0021-9304
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Poly[methacrylic acid‐grafted‐poly(ethylene glycol)] [P(MAA‐g‐EG)] is a complexation hydrogel molecularly designed for oral peptide delivery. In this work, the cytotoxicity and insulin‐transport enhancing effect of P(MAA‐g‐EG) microparticles on intestinal epithelial cells were evaluated using Caco‐2 cell monolayers. A series of P(MAA‐g‐EG) microparticles with different polymer compositions were prepared by a photo‐initiated free radical solution polymerization and subsequent pulverization. The hydrogel microparticles were preswollen in either Ca^2+^‐containing (CM+) or Ca^2+^‐free medium (CM−; pH 7.4) and applied to the apical side of the Caco‐2 monolayers. No significant cytotoxic effects, as determined by a calorimetric assay with P(MAA‐g‐EG) microparticles preswollen in the CM+, were observed at doses ranging from 3 to 31 mg/cm^2^ of cell monolayer. Transepithelial electrical resistance (TEER) measurements showed that the P(MAA‐g‐EG) microparticles induced a Ca^2+^ concentration‐dependent lowering in TEER values. The reduction effect in CM− media was greater than that in CM+ media (17 ± 2% reduction in CM+ and 45 ± 3% reduction in CM−, respectively). Insulin transport in the presence of the preswollen P(MAA‐g‐EG) microparticles was also strongly depended on the Ca^2+^ concentration in the medium. The respective estimated permeability for insulin alone and the insulin with hydrogels in CM+ were 0.77 and 1.16 × 10^−8^ cm/s, whereas those in CM− were 1.18 and 24.78 × 10^−8^ cm/s. The results demonstrate that the P(MAA‐g‐EG) hydrogel microparticles could be used as a cytocompatible carrier possessing the transport‐enhancing effect of insulin on the intestinal epithelial cells. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 609–617, 2003