## Abstract A stem cell has three important features. Firstly, the ability of selfβrenewal: making identical copies of itself. Secondly, multipotency, generating all the major cell lineages of the host tissue (in the case of embryonic stem cellsβpluripotency). Thirdly, the ability to generate/regen
Novel bioreactors for the culture and expansion of aggregative neural stem cells
β Scribed by Yuen Ling Ng; Howard A. Chase
- Publisher
- Springer
- Year
- 2007
- Tongue
- English
- Weight
- 310 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1615-7605
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Inoculation and growth conditions for the large-scale expansion of mammalian neural stem cells (NSC) have been determined. We examined suspension culture bioreactors of murine NSC, and concluded that the oxygen level should be kept high (20%), and the osmolarity of the medium should be kept low (bel
## Abstract __In vitro__ expansion of hematopoietic stem cells (HSCs) has been employed to obtain sufficient numbers of stem cells for successful engraftment after HSC transplantation. A threeβdimensional perfusion bioreactor system with a heparinβchitosan scaffold was designed and evaluated for it