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Novel Activity of UDP-Galactose-4-Epimerase for Free Monosaccharide and Activity Improvement by Active Site-Saturation Mutagenesis

โœ Scribed by Hye-Jung Kim; Sueng Yeun Kang; Jong Jin Park; Pil Kim


Publisher
Springer-Verlag
Year
2010
Tongue
English
Weight
171 KB
Volume
163
Category
Article
ISSN
0273-2289

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UDP-galactose 4-epimerase catalyzes the interconversion of UDP-galactose and UDP glucose. In the course of the reaction, the galacto-and glucopyranosyl rings undergo reversible oxidation to the 4-keto-glucopyranosyl ring by reaction with the enzyme-bound NAD + . The UDP-moiety of a substrate partici