[Novartis Foundation Symposia] Ciba Foundation Symposium - Growth Control in Cell Cultures (Ciba/Growth) || Studies of Serum Factors Required by 3T3 and SV3T3 Cells
β Scribed by Wolstenholme, G. E. W.; Knight, Julie
- Book ID
- 120164149
- Publisher
- John Wiley & Sons, Ltd.
- Year
- 2008
- Weight
- 745 KB
- Category
- Article
- ISBN
- 0700015175
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## Abstract Lactalbumin hydrolyzate (LH), a commercially prepared, enzymatic digest of a milk protein fraction, can partially replace the serum requirement of SV3T3 cells. In a low, but obligatory, background of calf serum (0.15% v/v), LH causes a large increase in the final cell density (5β10 x ov
## Abstract The growth requirements of SV40 transformed Balb/cβ3T3 cells have been studied in the absence of serum. For growth in serumβfree medium, the cells require (i) insulin, (ii) transferrin, and (iii) cisβunsaturated fatty acids added in combination with fatty acid free bovine serum albumin.
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