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Note on the paper of H. Venner and Ch. Zimmer

✍ Scribed by Gunther L. Eichhorn

Publisher
Wiley (John Wiley & Sons)
Year
1967
Tongue
English
Weight
41 KB
Volume
5
Category
Article
ISSN
0006-3525

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✦ Synopsis

COMMUNICATIONS TO THE EDITORS

Note on the Paper of H . Venner and Ch. Zimmer

The article referred to1 contains numerous references to "Eichhorn's buffer," which the authors supposed was used in our studies on metal ions as stabilizers or destabilizers of the deoxyribonncleic acid structure.2 Since we generally avoid the use of buffers in our Jtudies of metal-polynucleotide interactions, I reread the original paper on the siibjectz and found the following: I ' . . divalent ions have been added in 10-4M concentration to solutions of calf-thymus DNA (5 X lW5M in phosphate) in 5 X 10-3M sodiiim nitrate." What was meant was that the DNA concentration was 5 X 10-52CI (P); i.e., the DNA concentration was given in terms of its component nucleotide coilcentration, as is customary. Apparently the wording was interpreted by Drs. Venner and Zimmer to mean that inorganic phosphate was added to the system. I hope that this note will clear iip the conhision.

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