Northern blot analysis for detection and quantification of RNA in pancreatic cancer cells and tissues
✍ Scribed by Streit, Sylvia; Michalski, Christoph W; Erkan, Mert; Kleeff, Jörg; Friess, Helmut
- Book ID
- 109946559
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 339 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1750-2799
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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has been a gene of choice in Northern blot analyses as an internal RNA loading control. We have investigated the expression of GAPDH and 28S-ribosomal RNA (28S rRNA) genes in mouse 10T1/2 cells and in a variety of tumorigenic and highly malignant meta
## Abstract Pancreatic cancer is a highly aggressive type of malignancy and the prognosis for disease presenting typically at a late stage is extremely poor. A comprehensive understanding of its molecular genetics is required in order to develop new approaches to clinical management. To date, seria