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Normalization of the sleep–wake pattern and melatonin and 6-sulphatoxy-melatonin levels after a therapeutic trial with melatonin in children with severe epilepsy

✍ Scribed by J. Uberos; M. C. Augustin-Morales; A. Molina Carballo; J. Florido; E. Narbona; A. Muñoz-Hoyos

Book ID
111264808
Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
233 KB
Volume
50
Category
Article
ISSN
0742-3098

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Normalization of the sleep–wake pattern
Normalization of the sleep–wake pattern and melatonin and 6-sulphatoxy-melatonin levels after a therapeutic trial with melatonin in children with severe epilepsy
✍ J. Uberos; M. C. Augustin-Morales; A. Molina Carballo; J. Florido; E. Narbona; A 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB

**Abstract:** This study evaluated the sleep–wake pattern, plasma melatonin levels and the urinary excretion of its metabolite, 6‐sulphatoxy‐melatonin among children with severe epileptic disorders, before and after a therapeutic trial with melatonin. Ten paediatric patients, suffering from severe e

Saliva and serum samples were collected
Saliva and serum samples were collected from eight healthy volunteers every two hours during a 26-hour period. Melatonin concentrations were measured by radioimmunoassay after chloroform extraction using radioiodinated melatonin as a tracer. Five of the subjects had high serum melatonin levels at night (peak levels higher than 75 pg/ml); in three subjects the highest serum melatonin concentration was 20-40 pg/ml. All subjects had low levels (P <0.001, was obtained for all detectable value pairs (n= 73). The regression and correlation coefficients were almost equal for the peak values of melatonin and during the rising and descending phases of the secretion patterns. However, no significant correlation was found between low daytime salivary and serum concentrations when calculated separately. In the five high-secretors the melatonin levels in saliva reflected reliably the changes in serum, but in the three low-secretors the correlation between salivary and serum melatonin was not significant. The proportion of melatonin found in saliva decreased with increasing serum melatonin levels. Circadian rhythm parameters were estimated by single cosinor analysis. The acrophases did not differ significantly within a subject in the concomitant measurements of serum and salivary melatonin. The measurements of salivary melatonin levels seem valid for studies on melatonin rhythms, but the melatonin concentrations measured in saliva do not always consistently reflect the absolute concentrations in blood.
✍ Maija-Liisa Laakso; Tarja Porkka-Heiskanen; Aino Alila; Dag Stenberg; Gunnar Joh 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 622 KB