Normal breast epithelial cells produce interleukins 6 and 8 together with tumor-necrosis factor: Defective il6 expression in mammary carcinoma
✍ Scribed by Fulvio Basolo; Pier Giulio Conaldi; Lisa Fiore; Simonetta Calvo; Antonio Toniolo
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- French
- Weight
- 596 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Virtually pure primary cultures of normal mammary epithelial cells (MEC) obtained from healthy women were shown to release interleukin 6 and 8 (116, 118) and to produce a nonsecreted form of tumor-necrosis factor (TNF). No interferon (IFN), whether cu, f3 or y, or ILI-a or -f3 could be detected. Analysis of cellular RNA confirmed these findings and showed that MEC also express 116 receptor and TNF-a-related mRNAs. Epithelial cells were selectively stained by antibodies to 116, IL8 and TNF-a both in primary cultures and in the normal mammary gland. Samples of human milk contained sizable amounts of IL6, I18 and IFN-y; yet the liquid phase was consistently negative for other cytokines (i.e., TNF-a, IFN-a/-fi, ILI -a/+). Expression of 116 (but not of 118 and TNF-a) was abolished in ductal infiltrating carcinomas and greatly reduced in cultures of oncogene-transfected mammary cells, suggesting that alterations of IL6 expression are associated with pathogenesis in breast cancer.