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Normal and Perturbed Chinese Hamster Ovary Cells: Correlation of DNA, RNA, and Protein Content by Flow Cytometry

โœ Scribed by H. A. Crissman, Z. Darzynkiewicz, R. A. Tobey and J. A. Steinkamp


Book ID
124787755
Publisher
Rockefeller University Press.
Year
1985
Tongue
English
Weight
383 KB
Volume
101
Category
Article
ISSN
0021-9525

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Nuclei were isolated from various cell types including Chinese hamster ovary (CHO) and L1210 leukemia cell lines, primary cultures of fibroblasts, nonstimulated and stimulated human lymphocytes and mouse liver cells, by using different isolation techniques. The isolated nuclei were subsequently stai