Nonuniform field gel electrophoresis

✍ Scribed by C. Dennison; W.A. Lindner; N.C.K. Phillips

• Book ID: 102984652
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 618 KB
• Volume: 120
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90311-6

✦ Synopsis

The potential use of a nonlinear voltage gradient to linearize the logarithmic distribution of bands, which results from gel electrophoresis of protein mixtures in a linear voltage gradient, was investigated. Equations are developed to describe the behavior of sodium dodecyl sulfateprotein complexes undergoing electrophoresis in straight-sided conical and wedge-shaped gels and, conversely, equations are developed to describe the shape of the gels required to give an ideal proportionality between molecular weight and migration.