We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins. We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that con
โฆ LIBER โฆ
Nonsense suppressors partially revert the decrease of the mRNA level of a nonsense mutant allele in yeast
โ Scribed by D. Gozalbo; S. Hohmann
- Book ID
- 104717662
- Publisher
- Springer-Verlag
- Year
- 1990
- Tongue
- English
- Weight
- 261 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0172-8083
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โฆ Synopsis
suc2 degrees is a naturally occurring amber mutant allele of the yeast invertase structural gene SUC2. Strains carrying suc2 degrees had only 10% of the wild type invertase-specific mRNA level. Amber suppressors, which allowed suc2 degree strains to ferment sucrose caused an increase of the SUC-specific mRNA level.
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