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Nonsense suppressors partially revert the decrease of the mRNA level of a nonsense mutant allele in yeast

โœ Scribed by D. Gozalbo; S. Hohmann


Book ID
104717662
Publisher
Springer-Verlag
Year
1990
Tongue
English
Weight
261 KB
Volume
17
Category
Article
ISSN
0172-8083

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โœฆ Synopsis


suc2 degrees is a naturally occurring amber mutant allele of the yeast invertase structural gene SUC2. Strains carrying suc2 degrees had only 10% of the wild type invertase-specific mRNA level. Amber suppressors, which allowed suc2 degree strains to ferment sucrose caused an increase of the SUC-specific mRNA level.


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A method for isolating nonsense suppress
โœ Kennedy, Christina ;Crowlesmith, Ian ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Springer ๐ŸŒ English โš– 228 KB

We describe here the isolation of a mutant derivative of the drug resistance factor R1 (Meynell and Datta, 1966) that carries a nonsense mutation in a gene determining resistance to penicillins. We have used this mutant R1 to isolate derivatives of Escherichia coli and Klebsiella pneumoniae that con