Nonsense suppression context effects in Escherichia coli bacteriophage T4

✍ Scribed by Edelmann, Pat ;Martin, Robin ;Gallant, Jonathan

• Book ID: 104725839
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 211 KB
• Volume: 207
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00331625

✦ Synopsis

Nonsense suppression by supE44 has been examined in a collection of 14 T4 gene 22 and gene 23 UAG mutants, for which the precise gene location is known. In concordance with previous studies, UAG followed by a pyrimidine was inefficiently suppressed. However, among positions with similar 3' nucleotides, there was considerable variation in suppression efficiency. The competition between supE44 and Release Factor 1 (RF 1) was also investigated following the introduction of a multicopy RF 1 plasmid. An inverse relationship between the efficiency of suppression and RF 1 competition was observed.