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Nonradioactive telomerase activity assay by microchip electrophoresis: Privileges to the classical gel electrophoresis assay

✍ Scribed by Zhivko Zhelev; Rumiana Bakalova; Ashraf Ewis; Hideki Ohba; Mitsuru Ishikawa; Yoshinobu Baba

Book ID: 102185036
Publisher: John Wiley and Sons
Year: 2005
Tongue: English
Weight: 290 KB
Volume: 26
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200410435

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✦ Synopsis

Nonradioactive telomerase activity assay by microchip electrophoresis: Privileges to the classical gel electrophoresis assay

The present study accents on the privileges of microchip-based electrophoresis to the conventional gel electrophoresis in separation of telomerase repeat amplification protocol/polymerase chain reaction (PCR) ladder products obtained in telomerase-catalyzed reaction in cancer cells. We try to clarify the interpretation of the results obtained by both electrophoretic procedures and to avoid misinterpretation as a result of PCRdependent artefacts.

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