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Nonradioactive detection of telomerase activity using the telomeric repeat amplification protocol

โœ Scribed by Herbert, Brittney-Shea ;Hochreiter, Amelia E ;Wright, Woodring E ;Shay, Jerry W


Book ID
118676229
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
318 KB
Volume
1
Category
Article
ISSN
1750-2799

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Modified Telomeric Repeat Amplification
โœ Istvan Szatmari; Szilvia Tล‘kรฉs; Christopher B. Dunn; Thomas J. Bardos; Janos Ara ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 145 KB

A polymerase chain reaction (PCR)-based radioactive telomerase assay was developed in our laboratory which is quantitative and does not require electrophoretic evaluation (designated as TP-TRAP; it utilizes two reverse primers). The main steps of the assay include (1) extension of a 20-mer oligonucl